Jun 2013 - Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering

Haraguchi Y, Matsuura K, Shimizu T, Yamato M, Okano T. J Tissue Eng Regen Med. 2013; (e-pub ahead of print)

Human derived induced pluripotent stem cells have become a valuable and important source for therapeutic strategies in regenerative medicine. Classical expansion protocols rely on large culture dishes and the use of feeder cells (e.g. irradiated fibroblasts). This is quite a laborious procedure considering that for the treatment of a myocardial infarction approximately 500 densely grown 100 mm culture dishes would be required to provide the necessary mount of 1-2x 109 cardiac cells. The authors describe an alternative cell expansion method by applying rotary cell culture in spinner flasks, yielding up to 1x108 iPCSs, corresponding to 24 100 mm dishes. The cells grown under constant rotation form aggregates and stay undifferentiated with sustained pluripotency. Subjecting them afterwards to standard differentiation procedures yields electroactive cardiomyocytes with a conversion rate of 50-60%. Such new culture protocols will be essential in the field of regenerative medicine which will eventually help to provide the large amounts of cells required to achieve therapeutic success.

 

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