Gain more insight and longer biological functionality with InSphero's 3D liver microtissues
Rat liver microtissues: Co-cultures based on rat hepatocytes and other liver-specific cells
Launched in March 2011: A unique scaffold-free liver microtissues derived from primary rat liver (rLiMT), our best model ever for in-vitro liver toxicology!
NEW: Read our white paper on prediction of rare idiosyncratic toxicology effects with our rat-liver microtissue here.
With the goal of providing a more efficient, reliable and biomimetic alternative to current 2D sandwich cultures, this model resembles the cell composition of native liver and maintains hepatocyte-specific functionality over three weeks. Thus, chronic toxicology studies become possible in a standard 96-well format. The liver microtissue consists of selected hepatocytes in co-culture with liver-specific non-parenchymal cells, such as Kupffer macrophages, stellate cells and sinusoidal cells. This unique composition and the scaffold-free production at InSphero guarantee that the hepatocytes maintain a polarized structure and form bile canaliculi. The co-culture approach with Kupffer macrophages makes the liver microtissues an excellent model for studies involving inflammation-mediated liver hepatotoxicity. Moreover, secretion of albumin and urea is close to native liver – and stable over the full period of three weeks! Metabolic function of cytochrome P450 (CYP3A4) proteins is maintained perfectly, both basal levels and inducibility underline the high functionality of the new model.
Using the liver microtissues is simple as they are delivered in 96-well standard format plates with one tissue per well, ready for you to use. As they share the same endpoints and assay technology with standard 2D systems, an upgrade of any classical toxicology test to 3D is easy and straightforward. The patend-pending production technology at InSphero assures a size variation below 10% in diameter and a consistent cell density, which makes your tests campaigns more statistically relevant at low n.
If you are interested to learn more about our access routes to implement the technology in your lab, ranging from training and feasibility studies involving your compounds to off-the-shelf delivery of our rLiMT model, please contact our sales department.
Left image: Brightfield microscopy of rat liver microtissues showing the excellent size and density reproducibility. Right image: Morphological characterization by H&E staining or rat liver microtissues.