3D InSight™ Human Liver Microtissues

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Multi-donor |  Single-donor | Characterization Data

 

Delivering greater biological relevance and predictive power

3D InSight™ Human Liver Microtissues are a more sensitive model for DILI prediction with the specificity to accurately distinguish between known hepatotoxicants and structurally-related non-toxic analogs. Their multi-cellular composition and intact cytoarchitecture also provides an organotypic model for investigating mechanistic tox pathways, such as inhibition of bile acid secretion, idiosyncratic toxicity, and CYP-mediated toxicity.

  • Scaffold-free 3D models composed of primary human hepatocytes +/- Kupffer cells
  • Choice of pooled donor or single-donor hepatocytes for fit-for-purpose toxicity testing
  • High-sensitivity in vitro model for DILI testing that maintains high specificity
  • Suitable for long-term and idiosyncratic toxicity testing with repeat-dosing

 

Choose from Multi-donor or Single-donor models

 

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  • 3D InSight™ Microtissues display viability and functionality for more than 4 weeks in culture. Below, images of microtissues show consistent size over time. Intracellular ATP content was assessed using the CellTiter-Glo® Cell Viability Assay (Promega). Albumin secretion was measured with human albumin ELISA (Bethyl Laboratories).

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  • What is a microtissue2

    An intact bile canaliculi network expressing bile salt export pump (BSEP) protein (brown) in native liver (left) and recapitulated in 3D InSight™ Human Liver Microtissues at day 10 (right).

    Characterization 2

  • CYP activity & inducibility

    3D InSight™ MIcrotissues display sustained, inducible Cytochrome P450 enzyme activity persisting over 4 weeks in culture. Representative data from 3 donors depicted below.

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  • Sensitive, specific DILI models

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  • Detect idiosyncratic toxicity

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