Delivering greater biological relevance and predictive power
3D InSight™ Human Liver Microtissues are a more sensitive model for DILI prediction with the specificity to accurately distinguish between known hepatotoxicants and structurally-related non-toxic analogs. Their multi-cellular composition and intact cytoarchitecture also provides an organotypic model for investigating mechanistic tox pathways, such as inhibition of bile acid secretion, idiosyncratic toxicity, and CYP-mediated toxicity.
- Scaffold-free 3D models composed of primary human hepatocytes +/- Kupffer cells
- Choice of pooled donor or single-donor hepatocytes for fit-for-purpose toxicity testing
- High-sensitivity in vitro model for DILI testing that maintains high specificity
- Suitable for long-term and idiosyncratic toxicity testing with repeat-dosing
Choose from Multi-donor or Single-donor models
3D InSight™ Microtissues display viability and functionality for more than 4 weeks in culture. Below, images of microtissues show consistent size over time. Intracellular ATP content was assessed using the CellTiter-Glo® Cell Viability Assay (Promega). Albumin secretion was measured with human albumin ELISA (Bethyl Laboratories).
An intact bile canaliculi network expressing bile salt export pump (BSEP) protein (brown) in native liver (left) and recapitulated in 3D InSight™ Human Liver Microtissues at day 10 (right).
CYP activity & inducibility
3D InSight™ MIcrotissues display sustained, inducible Cytochrome P450 enzyme activity persisting over 4 weeks in culture. Representative data from 3 donors depicted below.
Sensitive, specific DILI models Detect idiosyncratic toxicity