11.05.2015 - InSphero & Promega User Group Seminar, Munich, Germany

Promega WS Munich

InSphero & Promega User Group Seminar

3D Cell Cultue Model Systems & Cell-based Assays for Use with 3D Cultures.


ZB Martinsried 
Zentralgebäude Konferenzraum
Kubus 1 und 2
Am Klopferspitz 19
82152 Planegg-Martinsried

Date and Time:

Monday, May 11, 2015
14:30 - 16:30


Dr. Simon Messner, Product Manager Microtissues

Dr. Terry Riss, Global Strategic Marketing Manager Cell Helth of Promega

Register here


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Microtissue models for drug de-risking


Cell-based assays have become an inherent part in drug discovery and
development to predict the in vivo response to biologicals and chemicals. The
better the in vivo system reflects the in vivo situation the better can a drug
being qualified. However, to gain the maximum benefit of in vivo cell cultures
for de-risking compounds cells have to be maintained in a format which reflects
in vivo cellular functionality, either animal or human, as closely as possible.
Organotypic 3D cell culture models can impact cell-based assays throughout
the drug development chain, including: (i) drug target validation, (ii) primary
and secondary screening set up’s, (iii) lead optimization and (iv) toxicological
profiling. With this objective, microtissue engineering strategies are being
exploited to further improve the predictive power of cell-based assays. Here
we present case studies how organotypic microtissue models can be applied
for target discovery, efficacy testing and toxicological profiling.




Application of in vitro assays to measure cellular response in 3D cell cultures

 The use of 3D cell cultures continues to emerge as improved in vitro models
that are more physiologically relevant and more predictive of cytotoxic
events in whole animals. Multicellular 3D culture systems containing more
than one cell type and exhibiting formation of a complex extracellular
matrix provide a challenge for assay chemistries originally designed for
measuring events from monolayers of cells. Critical factors to consider for
each model system and cell type include effective penetration of detection
reagents and/or complete lysis of microtissue structures using combinations
of detergent and physical disruption. We will present the approach used to
verify performance of the bioluminescent ATP detection assay for measuring
cell viability, a caspase assay for detecting apoptosis, and cell stress reporter
assays to detect mechanisms leading to cytotoxicity. Recommendations for
factors to consider when verifying performance of cell health assays on 3D
culture models will also be presented.





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