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InSphero to Present Novel 3D Human Liver Fibrosis Model at Annual Liver Meeting® in Washington, DC

InSphero to Present Novel 3D Human Liver Fibrosis Model at Annual Liver Meeting® in Washington, DC

New 3D InSight™ Human Liver Fibrosis Model represents a powerful tool for screening efficacy and safety of anti-fibrotic drugs in vitro. PDF Version

Schlieren, Switzerland -  Oct 18, 2017  IInSphero AG, the leading supplier of assay-ready 3D cell culture models for accelerating drug discovery and development, will present data characterizing and demonstrating the utility of its new 3D InSight™ Human Liver Fibrosis Model for screening efficacy of anti-fibrotic drugs.  The findings will be presented at oral and poster presentations this Friday at the annual meeting of the American Association for the Study of Liver Disease (AASLD) Conference in Washington, DC. 

Anti-fibrotic therapies aim to inhibit activation of hepatic stellate cell (HSCs), thereby preventing excessive accumulation of extracellular matrix (ECM) proteins, such as collagen, which can lead to cirrhosis and liver failure.  The InSphero 3D InSight™ Human Liver Fibrosis Model is unique in that it contains all liver cell types necessary to mimic fibrosis in vitro in a high throughput, screening-compatible platform.   

InSphero Chief Scientific Officer Dr. Patrick Guye says, “Our 3D human fibrosis model is composed of primary human liver cells, including HSCs, hepatocytes, Kupffer cells, and liver endothelial cells, all of which are required to accurately reflect the clinical fibrotic disease state in a cell culture model. Treatment of the model with TGF-b1, a known inducer of HSC activation, was shown to trigger strong induction of pro-fibrotic gene expression and ECM protein accumulation in the microtissue, resulting in collagen deposition and diminished hepatocyte function, both hallmarks of liver fibrosis. Even more promising, we were clearly able to demonstrate successful inhibition of disease progression on a phenotypic and transcriptional level using drugs known to interfere with pro-fibrotic signalling pathways.” 

Dr. Jan Lichtenberg, InSphero Chief Executive Officer and Co-founder, notes, “These findings support the power of our model to help drug discovery groups meet the demand for safer, more effective anti-fibrotic drugs. The initial feedback from beta testers within major pharmaceutical groups with fibrosis programs has been overwhelmingly positive.  Not only does the model overcome the lack of cellular complexity of current in vitro models, but being based on our pharma-validated Human Liver Microtissue platform, it can deliver valuable data on both the efficacy and safety of drug candidates, without the use of animal models.” 

AASLD Presentation Details
Friday, October 20: 12:00 - 1:30 PM
Abstract #385: Development and characterization of a novel in vitro human liver fibrosis model for efficacy testing of anti-fibrotic drugs based on 3D Human Liver Microtissues
Poster presented by Dr. Radina Kostadinova, InSphero AG

For more information about InSphero, visit www.insphero.com.

InSphero contacts

Dr. Randy Strube
Director of Global Marketing
Phone +1 800-779-7558 ext. 102
This email address is being protected from spambots. You need JavaScript enabled to view it.

Dr. Jan Lichtenberg
Chief Executive Officer and InSphero Co-founder
Phone +41 44 5150490
This email address is being protected from spambots. You need JavaScript enabled to view it.

 

About InSphero

InSphero sets the standard for in vitro testing of novel drugs in the pharmaceutical and biotechnology industry with comprehensive solutions that provide greater confidence in decision making. Its robust and highly physiologically relevant suite of 3D InSight™ Microtissues and Services are used by major pharmaceutical companies worldwide to increase efficiency in drug discovery and safety testing. InSphero patent-pending technologies and methods enable large-scale, reproducible production of scaffold-free 3D microtissues driven solely by cellular self-assembly. The company specializes in delivering assay-ready and custom 3D models derived from liver, pancreas, and tumor tissues, to provide unrivalled biological insight into liver toxicology, metabolic diseases (e.g., diabetes and liver diseases), and oncology. All InSphero microtissues are thoroughly validated to ensure the highest quality, certified for use in a variety of assays, and shipped globally to customers in a patented, easy-to-use spheroid-optimized platform, ready for research. Field application scientists and research staff with expertise in working with 3D models help ensure efficient integration and onsite training as needed. For customers who prefer an outsourcing strategy with fast turnaround, InSphero also offers contract research services utilizing their 3D microtissue models.

InSphero 3D InSight™ solutions drive significant findings in peer-reviewed journals, through collaborative industry initiatives such as EU-ToxRisk and HeCaToS, and have gained validation in the world’s largest government institutions and pharmaceutical, chemical and cosmetics companies.

Founded in 2009, the privately held company is headquartered in Schlieren, Switzerland with subsidiaries in the United States (Brunswick, ME) and Waldshut, Germany. It has been recognized for its scientific and commercial achievements with several national and international awards.

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Images

InSphero fibrosis model AASLD        Logo ohne Tagline ISGreen RGB

The 3D InSight™ Human Liver Fibrosis Model is a powerful in vitro tool used by pharmaceutical drug discovery groups to test the ability of novel anti-fibrotic drugs to prevent induced accumulation of collagen protein (green staining) within microtissues derived from primary human liver cells (blue DNA stain). 

Win an InSphero Microtissue Study! Join the sbv IMPROVER Network Verification Challenge 3

2017 PMI Network Verification Challenge "NVC3" Winner to Receive Service Project Conducted in 3D InSight™ Human Liver Microtissues 

Deadline:  December 31, 2017 

 sbv improver logo

 

About the NVC3 Challenge

The 3rd sbv IMPROVER Network Verification Challenge (“NVC3”) aims to verify and enhance three unpublished causal biological network models (one for each metabolic phase) that include the signaling pathways leading to the activation of enzymes involved in the three phases of liver xenobiotic metabolism.

The NVC3 is expected to increase the networks' value and promote their use in research applications, including:

  • Toxicology: to predict adverse outcomes and gain mechanistic understanding in response to exposures;
  • Pharmacology: to establish drug mode of action and predict adverse drug effect;
  • Personalized medicine: patient diagnosis and prediction of treatment outcome;
  • Basic research: to build biological hypotheses from high-dimensional data sets such as those derived from microarray profiling studies.

 

Why should you participate in the NVC3?

Participants are part of a scientific community curating networks that describe key biological processes that may be key for the fields of systems biology and toxicology.  In addition, the winner of the Challenge will be awarded a complementary study conducted in 3D InSight™ Human Liver Microtissues, contributing data generated in more physiologically relevant 3D models to the Network.  Travel grants and gift cards are also available as secondary prizes.  

 

It's not too late to participate!  

Visit the NVC3 website for an overview of the models, to learn more about the Challenge, or Register to Participate.  

 

About the sbv IMPROVER project

The sbv IMPROVER project, the websites and the Symposia are part of a collaborative project designed to enable scientists to learn about and contribute to the development of a new crowd sourcing method for verification of scientific data and results. The project is led and funded by Philip Morris International. The current challenges, website and biological network models were developed and are maintained as part of a collaboration with Selventa, Douglas Connect, SBX, OrangeBus and ADS. For more information on Philip Morris International’s research, please visit www.pmiscience.com.

Comprehensive Study by AstraZeneca and Genentech Confirms Superiority of InSphero Human Liver Microtissues for Prediction of Drug-Induced Liver Injury

Comprehensive Study by AstraZeneca and Genentech Confirms Superiority of InSphero Human Liver Microtissues for Prediction of Drug-Induced Liver Injury

Archives of Toxicology publication reveals microtissues are twice as sensitive as 2D primary human hepatocytes (PHH) for predicting hepatotoxicity of 110 clinically defined DILI compounds. PDF Version

Schlieren, Switzerland -  June 29, 2017  In the most comprehensive evaluation of 3D spheroid hepatic cultures conducted to date, a publication co-authored by lead toxicologists and drug safety experts at AstraZeneca and Genentech revealed InSphero 3D InSight™ Human Liver Microtissues to be a more sensitive and highly specific in vitro model for predicting drug-induced liver injury (DILI) compared to 2D models. The study entitled, “Utility of spherical human liver microtissues for prediction of clinical drug-induced liver injury,” was published online Tuesday, June 13 in the journal Archives of Toxicology. Dr. William Proctor, Head of Investigative Toxicology at Genentech, and Alison Foster, Senior Scientist in the Drug Safety and Metabolism group at AstraZeneca, were co-first authors on the publication.

2D PHH assays have been the “gold-standard” model used for DILI testing, yet DILI still remains one of the leading sources for drug attrition, black box warnings, and post-market withdrawal of drugs. Thus, there is a growing need for better predictive tools to assess liver injury earlier in the drug development process. The authors note in the paper that, “3D models have shown promise in small sets of DILI compounds, but a more comprehensive retrospective study of DILI positive and negative drugs to test the predictive power of 3D models head to head with 2D PHH assays had not been performed.”

The study determined that regardless of the threshold used, “Human Liver Microtissues demonstrated increased sensitivity in identifying known hepatotoxicants versus PHH, while specificity was consistent across both assays.” Microtissues also, “outperformed PHH in correctly classifying hepatotoxicants from different pharmacological classes of molecules.” Furthermore, the authors found that 3D microtissues displayed sufficient structural and functional characteristics to warrant exploratory studies to see if novel biomarkers used in the clinic could also be detected in vitro. Findings revealed that despite each microtissue consisting of ~1000 cells, the mechanistic and exploratory hepatotoxicity biomarkers miR-122, HMGB1, and α-GST could be detected in the culture supernatants, demonstrating that, “3D liver microtissues have the potential to recapitulate in vivo findings in vitro.”

“This landmark study provides convincing, independent evidence that InSphero liver microtissues can help identify hepatotoxic drugs with greater sensitivity without sacrificing specificity, says InSphero Chief Executive Officer and Co-founder Dr. Jan Lichtenberg. “Having two of the world’s leading pharmaceutical companies collaborate to produce this thorough validation further confirms that our 3D models not only better reflect in vivo biology, but also deliver the reproducibility and scalability required to meet the early stage screening demands of large pharma. Our assay-ready 3D InSight™ models offer a cost-effective, turnkey solution that gives toxicologists greater confidence when characterizing risk in lead compound sets.”

3D InSight™ Human Liver Microtissues are a standardized primary human liver model developed by InSphero that display enhanced liver phenotype, metabolic activity, and stability over 28 days in culture, enabling long-term drug exposure studies not attainable with 2D hepatic models.

For more information about InSphero 3D InSight™ Liver Microtissues and applications, visit www.insphero.com

InSphero contacts

Dr. Randy Strube
Director of Global Marketing
Phone +1 800-779-7558 ext. 102
This email address is being protected from spambots. You need JavaScript enabled to view it.

Dr. Jan Lichtenberg
Chief Executive Officer and InSphero Co-founder
Phone +41 44 5150490
This email address is being protected from spambots. You need JavaScript enabled to view it.

 

About InSphero

InSphero sets the standard for in vitro testing of novel drugs in the pharmaceutical and biotechnology industry with comprehensive solutions that provide greater confidence in decision making. Its robust and highly physiologically relevant suite of 3D InSight™ Microtissues and Services are used by major pharmaceutical companies worldwide to increase efficiency in drug discovery and safety testing. InSphero patent-pending technologies and methods enable large-scale, reproducible production of scaffold-free 3D microtissues driven solely by cellular self-assembly. The company specializes in delivering assay-ready and custom 3D models derived from liver, pancreas, and tumor tissues, to provide unrivalled biological insight into liver toxicology, metabolic diseases (e.g., diabetes and liver diseases), and oncology (with a focus on immuno-oncology). All InSphero microtissues are thoroughly validated to ensure the highest quality, certified for use in a variety of assays, and shipped globally to customers in a patented, easy-to-use spheroid-optimized platform, ready for research. Field application scientists and research staff with expertise in working with 3D models help ensure efficient integration and onsite training as needed. For customers who prefer an outsourcing strategy with fast turnaround, InSphero also offers contract research services utilizing their 3D microtissue models.

InSphero 3D InSight™ solutions drive significant findings in peer-reviewed journals, through collaborative industry initiatives such as EU-ToxRisk and HeCaToS, and have gained validation in the world’s largest government institutions and pharmaceutical, chemical and cosmetics companies.

Founded in 2009, the privately held company is headquartered in Schlieren, Switzerland with subsidiaries in the United States (Brunswick, ME) and Waldshut, Germany. It has been recognized for its scientific and commercial achievements with several national and international awards.

Follow us on Twitter and and LinkedIn.

 

Images

Liver Microtissue        Logo ohne Tagline ISGreen RGB

InSphero 3D InSight™ Liver Microtissues help drug discovery and development groups predict drug-induced liver injury (DILI) with greater confidence, providing more relevant and reliable 3-dimensional cell based models for in vitro drug safety testing.

ADA Presentations Highlight Superior Functionality of InSphero Standardized Islet Microtissues for Diabetes Drug Screening

ADA Presentations Highlight Superior Functionality of InSphero Standardized Islet Microtissues for Diabetes Drug Screening

ETH Zurich researchers present single-islet study using novel microfluidic setup to display in vivo-like temporal insulin response to high glucose. PDF Version

Schlieren, Switzerland -  June 19, 2017  InSphero AG highlighted unique advantages of its physiologically relevant 3D InSight™ Islet Microtissue Platform to drive more efficient development of anti-diabetic drugs at the American Diabetes Association 77th Scientific Sessions in San Diego, CA. Also, a pioneering study describing a microfluidic single islet perifusion platform, developed by researchers at the ETH Zurich, was presented during open and moderated poster sessions held during the conference.

Dr. Burcak Yesildag, islet product manager at InSphero and presenter of the study at ADA says, “Loss of first phase, reduction in the second phase and impairment of the oscillatory pattern of insulin secretion are characteristic features of type 2 diabetes. Therefore, study of dynamic insulin release with in vitro perifusion assays is a crucial component of pancreatic islet research. However, inherent heterogeneity in islet size and composition necessitates pooling of multiple islets for each experimental condition, which entails inefficient use of donor material and decreased experimental resolution due to uncoordinated inter-islet function.”

InSphero 3D InSight™ Islet Microtissues - uniform in size, cellular composition and function – were combined with a novel microfluidic system engineered by Dr. Andreas Hierlemann’s research group at the Swiss Federal Institute of Technology (ETH). The microfluidic system facilitates rapid glucose switches and short sampling intervals, making it possible to study dynamic insulin secretion at the single-islet level in vitro.

Says Yesildag, “ETH observed an organotypic biphasic and pulsatile secretion of insulin from our islet microtissues which was physiologically modulated by various insulin secretagogues. The results very closely mirrored the magnitude and frequency of the response seen in humans. This collaboration provides further evidence that the in vivo-like functionality of our islet model makes it a powerful new tool for islet researchers to help accelerate anti-diabetic drug development.”

3D InSight™ Islet Microtissues are a standardized primary islet model developed by InSphero that display organotypic function with an average 13-fold increase in glucose-stimulated insulin secretion (GSIS) for at least 28 days in culture.

For more information about InSphero 3D InSight™ Islet Microtissues, visit www.insphero.com or watch the video.

InSphero contacts

Dr. Randy Strube
Director of Global Marketing
Phone +1 800-779-7558 ext. 102
This email address is being protected from spambots. You need JavaScript enabled to view it.

Dr. Patrick Guye
Chief Scientific Officer
Phone +41 44 5150490
This email address is being protected from spambots. You need JavaScript enabled to view it.

 

About InSphero

InSphero sets the standard for in vitro testing of novel drugs in the pharmaceutical and biotechnology industry with comprehensive solutions that provide greater confidence in decision making. Its robust and highly physiologically relevant suite of 3D InSight™ Microtissues and Services are used by major pharmaceutical companies worldwide to increase efficiency in drug discovery and safety testing. InSphero patent-pending technologies and methods enable large-scale, reproducible production of scaffold-free 3D microtissues driven solely by cellular self-assembly. The company specializes in delivering assay-ready and custom 3D models derived from liver, pancreas, and tumor tissues, to provide unrivalled biological insight into liver toxicology, metabolic diseases (e.g., diabetes and liver diseases), and oncology (with a focus on immuno-oncology). All InSphero microtissues are thoroughly validated to ensure the highest quality, certified for use in a variety of assays, and shipped globally to customers in a patented, easy-to-use spheroid-optimized platform, ready for research. Field application scientists and research staff with expertise in working with 3D models help ensure efficient integration and onsite training as needed. For customers who prefer an outsourcing strategy with fast turnaround, InSphero also offers contract research services utilizing their 3D microtissue models.

InSphero 3D InSight™ solutions drive significant findings in peer-reviewed journals, through collaborative industry initiatives such as EU-ToxRisk and HeCaToS, and have gained validation in the world’s largest government institutions and pharmaceutical, chemical and cosmetics companies.

Founded in 2009, the privately held company is headquartered in Schlieren, Switzerland with subsidiaries in the United States (Brunswick, ME) and Waldshut, Germany. It has been recognized for its scientific and commercial achievements with several national and international awards.

Follow us on Twitter and and LinkedIn.

 

Images

Islet no background        Logo ohne Tagline ISGreen RGB

InSphero 3D InSight™ Islet Microtissues help drug discovery and development groups identify more effective drugs for diabetes by providing more relevant and reliable 3-dimensional cell based models for in vitro efficacy testing.

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