Fast track your evaluation of 3D InSight™ Human Liver Microtissues for drug induced liver injury (DILI) prediction
This service is designed to enable you to quickly assess the predictive power of our 3D in vitro liver models for drug safety testing without the challenges and expense typically associated with new model development, evaluation, and standardization. It combines the convenience of outsourcing with a highly optimized, proven protocol for DILI compound classification.
- Test 6 compounds in physiologically relevant liver model composed of pooled multi-donor primary human hepatocytes co-cultured with Kupffer cells
- Achieve maximum sensitivity and specificity with a 14 day repeat dosing regimen and single ATP endpoint
- Receive IC50 values for your test compounds plus Chlorpromazine positive control in just 3 weeks
- Compare results to classification data from historical reference compound data set
Service at a Glance
The first step toward 3D model integration. This service enables you to conduct a controlled experiment to better assess how you can efficiently integrate 3D models into your safety workflow without launching a major new development effort. Send us 6 compounds of interest, we'll run the experiments and classify their potential for DILI.
Our DILI Evaluation Service 14 day repeat-dosing protocol
Our organotypic 3D liver model. 3D InSight™ Human Liver Microtissues are composed of pooled multi-donor primary human hepatocytes (PHH) co-cultured with Kupffer cells. They display polarized, cuboidal shaped hepatocytes, integration of CD68+ Kupffer cells, and formation of functional bile canaliculi (BSEP shown), representing intact cholestatic and inflammatory mechanisms of DILI.
A proven model and protocol for DILI prediction, featuring:
- Organotypic, genetically diverse primary hepatocyte model that mitigates potentially confounding responses resulting from single-donor CYP polymorphisms
- Longevity > 4 weeks in vitro enables repeat dosing experiments
- Co-culture with non-parenchymal cells, including Kupffer cells
- No bioartificial matrices
- Optimized Promega CellTiter-Glo® assay
Discover the benefits of 14 day dosing protocol:
- Chronic exposure to test chemicals elicits different biological effects than short-term high dose exposures
- Primary, secondary and tertiary effects on stress pathways and adaptive responses are captured
- Predictive value increases with exposure time, with highest sensitivity towards potential hepatotoxicants, while maintaining high specificity