3D InSight™ Cell-Culture Media

Optimized media for 3D cell culture – ready to use!

  • Safeguard your 3D microtissues in culture for weeks
  • Optimized for assay-ready 3D InSight™ Microtissues
  • Specialized media for tumor spheroid aggregation and maintenance
  • Ready-to-use, no mixing and preparation required

 

  • Catalog # Description
    CS-07-001-xx 3D InSight™ Human Liver Maintenance Medium - TOX (for Toxicology studies)*
    CS-07-001a-xx 3D InSight™ Human Liver Maintenance Medium - AF (advanced formulation)*
    CS-07-001b-xx 3D InSight™ Human Liver Maintenance Medium - INF (for inflammatory tox applications)*
    CS-07-002-xx 3D InSight™ Rat Liver Maintenance Medium*
    CS-07-005-xx 3D InSight™ Human Islets Maintenance Medium*
    CS-07-006-xx 3D InSight™ Rat Islets Maintenance Medium*
    CS-07-100-01 3D InSight™ HepG2 Microtissue Maintenance Medium*
    CS-07-101-xx 3D InSight™ Cell Line Maintenance Medium


    NB: „xx" is the volume code: -01 for 500 ml and -02 for 250 ml. Please also inquire about customer-specific formulations.

    * only available together with 3D InSightTM Microtissues

  • Tailor-made media for reliable cultivation of 3D microtissues

    Cells cultured in scaffold-free spheroids – such as InSphero's assay-ready 3D InSight™ Microtissues – maintain their tissue-specific phenotype over long periods in culture. This is a key reason why 3D InSight™ Microtissues represent one of the most powerful in vitro systems for predictive testing of compound efficacy and toxicity. To ensure consistent and successful maintenance of 3D microtissues in your laboratory, InSphero offers a range of cell-culture media optimized for 3D spheroids. For optimal cultivation of microtissues, InSphero's scientists have developed enhanced medium compositions capable of maintaining microtissue functionality over long cultivation periods. The media are freshly prepared at InSphero and shipped ready to use at 4°C.

    3D InSight™ Liver Maintenance Media

    3D InSight™ Liver Maintenance Media are available for 3D InSight™ Human and Rat Liver Microtissues made from primary hepatocytes. The medium has been formulated to optimally nourish these demanding cells in their 3D microenvironment for consistent stability and long-term culture.

    • Serum-free media for perfect compatibility with endpoint assays (e.g. LDH)
    • Hormonally defined
    • Enables long-term culture of hepatic 3D InSight™ Microtissues up to 28 days
    • Assures functionality and microtissue stability
    • Shelf life: 2 months for human, 1 month for rat
    • Delivered ready to use, shipped at 4°C
    • 0.2-μm filtered
    • Available in 125, 250 and 500 mL bottles

    3D InSight™ Islet Maintenance Media

    3D InSight™ Islet Maintenance Media are available for 3D InSight™ Human and Rat Pancreatic Islet Microtissues.  The medium has been formulated and optimized for culture with sustained viability and glucose responsiveness. 

    3D InSight™ Human Islet Maintenance Medium

    • Enables long-term culture of 3D InSight™ Human Pancreatic Microislets up to 28 days
    • Shelf life: 1 month
    • Delivered ready to use, shipped at 4°C
    • 0.2-μm filtered
    • Available in 250 and 500 mL bottles

     3D InSight™ Rat Islet Maintenance Medium

    • Enables long-term culture of 3D InSight™ Rat Pancreatic Microislets up to 28 days
    • Shelf life: 1 month
    • Delivered ready to use, shipped at 4°C
    • 0.2-μm filtered
    • Available in 250 and 500 mL bottles

     

    3D InSight™ Cell Line Maintenance Medium

    Especially in oncology applications, cell lines are a convenient and reproducible cell source to create 3D microtissue spheroids. The 3D InSight™ Cell Line Maintenance Medium can be used for most cell lines. It encourages cellular re-aggregation, maintains spheroid phenotype and supports tumor cell growth. Based on carefully validated serum lots, the medium is not only an optimal choice for 3D microtissue maintenance, but also for in-house microtissue production using InSphero's GravityPLUS™ 3D Cell-Culture Platform.

    • Serum lots extensively tested and validated for 3D cell culture
    • Enables long-term culture of growing cell-line microtissues and consistent morphology
    • Shelf life: 3 months
    • Delivered ready to use, shipped at 4°C
    • 0.2-μm filtered
  • Support

    Please send your inquiry to This email address is being protected from spambots. You need JavaScript enabled to view it. or contact This email address is being protected from spambots. You need JavaScript enabled to view it. for pricing/ quotation matters.

    Documents and downloads

    Product brochure

Company video

company-video-inspheor