InSphero offers advanced, pre-validated 3D liver models engineered to help you assess toxicity risk earlier in the drug discovery process, and screen drugs for metabolic diseases of the liver, such as fibrosis.
De-risk with Greater Confidence
3D InSight™ Liver Microtissues are standardized 3D liver models, produced using primary hepatocytes and non-parenchymal liver cells. These advanced liver models accurately reflect the structure and biology of in vivo liver, delivering greater confidence in decision-making based on in vitro cell-based assays for liver injury and drug efficacy testing.
Explore key mechanisms of healthy and diseased liver function
Our liver microtissues are comprised of primary human or animal hepatocytes and non-parenchymal liver cell types, such as Kupffer cells and liver endothelial cells, resulting in native-tissue-like functionality. They exhibit albumin secretion, polarized cellular organization, robust cytochrome P450 activity, high mitochondrial function, and bile-acid transport to bile canaliculi. This long-lived model improves sensitivity for DILI prediction by enabling long-term drug exposure and by including a physiologically relevant ratio of primary hepatocytes to non-parenchymal cells.
Improve sensitivity for DILI prediction by employing long-term drug exposure
Guaranteed viability for 28 days in most of our liver models allows highly reproducible assessment of both chronic and short-term drug exposure without the use of animals. Learn how you can detect DILI earlier and read the Arch Toxicol Paper
Ensure reproducibility and continuity in longitudinal and multi-site studies
Order standardized, pre-qualified liver models, certified for liver toxicology applications, such as DILI prediction, that are available for delivery direct to your lab when you're ready to start experiments. Check our production schedule
Human and Animal-derived Liver Microtissues
"Making primary hepatocyte-derived liver microtissues containing the appropriate number of non-parenchymal cells is complicated. In a European consortium, which performed a ring trial with 3D liver models, multiple labs failed to produce liver models themselves despite all using the same protocol and donor cells. When using InSphero’s assay-ready 3D InSight™ Microtissues, you avoid high upfront costs and frustrations, so you can focus on the most important part: generating reproducible data with a robust 3D model you can rely on."
RADINA KOSTADINOVA, PHD
3D InSight™ Liver Microtissue Product Manager
Tier 1: Short-term (max. 7 days)
Tier 2: Long-term (14 days)
In Vitro Toxicology
14 Day Hepatotoxicity
Custom Mechanistic & Investigative Studies
Debating whether to make or buy your liver microtissues? Read this first.
InSphero 3D InSight™ Liver Microtissues are next-generation 3D models for DILI and investigative toxicity testing. Using the 3D Select™ Process, we ensure only the most viable hepatocytes incorporate into our microtissues, improving viability and organotypic morphology. When compared head-to-head to conventional production methods, there is no comparison. See the results in this White Paper: