3D InSight™ Human Liver Microtissues

3D Liver models made with primary human hepatocytes

3D Human Liver Model made with primary human hepatocytes

Characterization Data

Obtain greater biological relevance and predictive power

3D InSight™ Human Liver Microtissues deliver high biological relevance due to the tight intercellular interactions between primary human hepatocytes, establishing a polarized structure with active uptake and secretion pathways including bile canaliculi. The co-culture with primary Kupffer cells enables modeling of inflammatory reactions. This human liver model not only provides confidence in the obtained data because of its multicellular primary cell composition, but also because the most relevant toxicity pathways typically observed in vivo are reflected in this model, including: cytochrome metabolism, mitochondrial function, hepatocellular efflux and interaction with non-parenchymal liver cells. The result is 3D InSight™ Human Liver Microtissues: a sensitive model for DILI prediction with the specificity to accurately distinguish between known hepatotoxicants and structurally-related non-toxic analogs. Their properties also make them a suitable model for investigating mechanistic tox pathways, such as inhibition of bile acid secretion, inflammation-mediated toxicity, and CYP-mediated toxicity.

  • Scaffold-free 3D spheroid models composed of primary human hepatocytes +/- Kupffer cells
  • Choice of pooled donor or single-donor hepatocytes for fit-for-purpose toxicity testing
  • Predictive DILI model, which detects up to 50% more true DILI compounds than 2D culture, while maintaining high specificity for non-DILI compounds
  • Suitable for long-term and idiosyncratic toxicity testing with repeat-dosing

learn more about Human Islet Microtissues    quote

Just getting started with 3D DILI testing? Evaluate now.

  • InSphero 3D InSight™ Human Liver Microtissues are produced weekly and regularly shipped to customers in the United States and Europe. Place your order on or before Tuesday at 6 PM CET (12 PM EST) to have your microtissues ship the following week.

    For pricing information and shipping costs, please request a quote, indicating the desired model system, the number of plates needed, and the preferred ship date. For more detailed information about the order and shipment dates for all InSphero microtissue products, please see our quarterly production schedule.

    3D InSight™ Human Liver Microtissues
    Production lead time: 1 week

    Catalog # Description Order by ► Ships on: US | EU
    MT-02-302-04
    3D InSight™ Human Liver Microtissues (multi-donor hepatocytes, co-culture with Kupffer cells), 96x

    Dec  13  ►  Dec 20  |  Dec 21
    Jan  03   ►  Jan 10  |  Jan 11
    Jan  10   ►  Jan 17  |  Jan 18
    Jan  17   ►  Jan 24  |  Jan 25
    Jan  24   ►  Jan 31  |  Feb 01
    Jan  31   ►  Feb 07  |  Feb 08
    Feb  07   ►  Feb 14  |  Feb 15
    Feb  14   ►  Feb 21  |  Feb 22
    Feb  21   ►  Feb 28  |  Mar 01
    Feb  28   ►  Mar 07  |  Mar 08
    Mar  07   ►  Mar 14  |  Mar 15
    Mar  14   ►  Mar 21  |  Mar 22
    Mar  21   ►  Mar 28  |  Mar 29
    Mar  28   ►  Apr 04  |  Apr 05
    Apr  04   ►  Apr 11  |  Apr 12
    Apr  11   ►  Apr 18  |  Apr 19
    Apr  18   ►  Apr 25  |  Apr 26

    MT-02-302-01
    3D InSight™ Human Liver Microtissues (multi-donor hepatocytes, monoculture), 96x
    KT-02-121-14 3D InSight™ DILI Evaluation Kit (6 plates of 96x Multi-donor microtissues, media, ATP assay, and reference compounds)
    MT-02-002-04 3D InSight™ Human Liver Microtissues (single-donor hepatocytes, co-culture with Kupffer cells), 96x
    MT-02-002-01 3D InSight™ Human Liver Microtissues (single-donor hepatocytes, monoculture), 96x
    CS-07-001-01 Maintenance Medium TOX*
    (for tox testing), 500 mL
     
    CS-07-001a-01 Maintenance Medium – AF*
    (for basic cultivation), 500 mL
     
    CS-07-001b-01 Maintenance Medium – INF*
    (for inflammatory tox testing), 500 mL
     

     For volume discounts (starting at two plates) and shipping cost, please contact our sales staff to request a quote.

    * Available only when purchased with 3D InSight™ Human Liver Microtissues.

  • 3D InSight™ Microtissues display viability and functionality for more than 4 weeks in culture. Below, images of microtissues show consistent size over time. Intracellular ATP content was assessed using the CellTiter-Glo® Cell Viability Assay (Promega). Albumin secretion was measured with human albumin ELISA (Bethyl Laboratories).

    3D Human Liver Model with primary human hepatocytes
long-term viability

  • 3D Human Liver Model with primary human hepatocytes
versus native human liver

    An intact bile canaliculi network expressing bile salt export pump (BSEP) protein (brown) in native liver (left) and recapitulated in 3D InSight™ Human Liver Microtissues at day 10 (right).

    3D Human Liver Model with primary human hepatocytes
structure IHC

  • CYP activity & inducibility

    3D InSight™ MIcrotissues display sustained, inducible Cytochrome P450 enzyme activity persisting over 4 weeks in culture. Representative data from 3 donors depicted below.

     3D Human Liver Model with primary human hepatocytes
CYP activity
  • Sensitive, specific DILI models

    3D Human Liver Model with primary human hepatocytes
sensitivity and specificity
  • Detect idiosyncratic toxicity

    3D Human Liver Model with primary human hepatocytes
Inflammatory Tox

 

 

Company video

company-video-inspheor