3D Liver models made with primary human hepatocytes
Obtain greater biological relevance and predictive power
3D InSight™ Human Liver Microtissues deliver high biological relevance due to the tight intercellular interactions between primary human hepatocytes, establishing a polarized structure with active uptake and secretion pathways including bile canaliculi. The co-culture with primary Kupffer cells enables modeling of inflammatory reactions. This human liver model not only provides confidence in the obtained data because of its multicellular primary cell composition, but also because the most relevant toxicity pathways typically observed in vivo are reflected in this model, including: cytochrome metabolism, mitochondrial function, hepatocellular efflux and interaction with non-parenchymal liver cells. The result is 3D InSight™ Human Liver Microtissues: a sensitive model for DILI prediction with the specificity to accurately distinguish between known hepatotoxicants and structurally-related non-toxic analogs. Their properties also make them a suitable model for investigating mechanistic tox pathways, such as inhibition of bile acid secretion, inflammation-mediated toxicity, and CYP-mediated toxicity.
- Scaffold-free 3D spheroid models composed of primary human hepatocytes +/- Kupffer cells
- Choice of pooled donor or single-donor hepatocytes for fit-for-purpose toxicity testing
- Predictive DILI model, which detects up to 50% more true DILI compounds than 2D culture, while maintaining high specificity for non-DILI compounds
- Suitable for long-term and idiosyncratic toxicity testing with repeat-dosing
InSphero 3D InSight™ Human Liver Microtissues are produced regularly and shipped to customers in the United States and Europe. Place your order on or before Tuesday at 6 PM CET (12 PM EST) to have your microtissues ship according to the shedule below.
For pricing information and shipping costs, please request a quote, indicating the desired model system, the number of plates needed, and the preferred ship date. For more detailed information about the order and shipment dates for all InSphero microtissue products, please see our quarterly production schedule.
3D InSight™ Human Liver Microtissues
Production lead time: 1 week
Catalog # Description Order by ► Ships on: US | EU MT-02-302-04 3D InSight™ Human Liver Microtissues (multi-donor hepatocytes, co-culture with Kupffer cells), 96x
Jul 11 ► Jul 18 | Jul 19
Jul 18 ► Jul 25 | Jul 26
Aug 01 ► Aug 08 | Aug 09
Aug 15 ► Aug 22 | Aug 23
Aug 29 ► Sep 5 | Sep 6
Sep 12 ► Sep 19 | Sep 20
Sep 26 ► Oct 03 | Oct 04
Oct 10 ► Oct 17 | Oct 18
Oct 24 ► Oct 31 | Nov 1
MT-02-302-01 3D InSight™ Human Liver Microtissues (multi-donor hepatocytes, monoculture), 96x KT-02-121-14 3D InSight™ DILI Evaluation Kit (6 plates of 96x Multi-donor microtissues, media, ATP assay, and reference compounds) MT-02-002-04 3D InSight™ Human Liver Microtissues (single-donor hepatocytes, co-culture with Kupffer cells), 96x MT-02-002-01 3D InSight™ Human Liver Microtissues (single-donor hepatocytes, monoculture), 96x CS-07-001-01 Maintenance Medium – TOX*
(for tox testing), 500 mL
CS-07-001a-01 Maintenance Medium – AF*
(for basic cultivation), 500 mL
CS-07-001b-01 Maintenance Medium – INF*
(for inflammatory tox testing), 500 mL
For volume discounts (starting at two plates) and shipping cost, please contact our sales staff to request a quote.
* Available only when purchased with 3D InSight™ Human Liver Microtissues.
3D InSight™ Microtissues display viability and functionality for more than 4 weeks in culture. Below, images of microtissues show consistent size over time. Intracellular ATP content was assessed using the CellTiter-Glo® Cell Viability Assay (Promega). Albumin secretion was measured with human albumin ELISA (Bethyl Laboratories).
An intact bile canaliculi network expressing bile salt export pump (BSEP) protein (brown) in native liver (left) and recapitulated in 3D InSight™ Human Liver Microtissues at day 10 (right).
CYP activity & inducibility
3D InSight™ MIcrotissues display sustained, inducible Cytochrome P450 enzyme activity persisting over 4 weeks in culture. Representative data from 3 donors depicted below.
Sensitive, specific DILI models Detect idiosyncratic toxicity