3D InSight™ Liver Microtissues for Toxicology (New)

 

More sensitive, more predictive 3D liver models for DILI testing

 

3D liver models derived from primary human hepatocytes (PHH) and non-parenchymal cells (NPCs) are a more biologically relevant and versatile model system for the study of drug-induced liver injury (DILI), drug targets, and liver disease. Even following published methods, it can take years and considerable expense to develop and implement a single 3D liver model. Discover how our 3D Select™ Production Process sets InSphero apart, and bring next-generation 3D InSight™ Liver Microtissues into your lab immediately. 


3D Liver Models

Properties

  • Self-assembly-driven aggregation
  • Organotypic liver structure and ECM
  • Long-lived in vitro model
  • Robust, inducible CYP expression
  • Amenable to multiple species

Advantages

  • More sensitive, predictive DILI model
  • Mechanistically accurate
  • Maximal endpoint flexibility
  • Useful for DILI, MOA, and Translational Tox

Improved biological relevance
• • •

Liver Model Comparison

3D InSight™ Liver Microtissues
Self-made PHH spheroids

3D Select™
Production Process

Enhancements

  • Thorough donor selection and procurement
  • Unique hepatocyte preselection step
  • Bioengineered co-cultures with hepatocytes and NPCs
  • Scaffold-free hanging drop technology
  • Best-in-class quality control

Advantages

  • Maximum donor compatibility
  • Superior morphology
  • Higher viability
  • Improved functionality


"Next-gen"
microtissues

• • •

Liver Model Comparison

3D InSight™ Liver Microtissues
Self-made PHH spheroids


Assay-ready Format

What's included

  • User-friendly 96-well GravityTRAP™ plate
  • Regular production and worldwide delivery
  • Standardized 3D models
  • Optimized culture and assay media
  • First class customer support

Advantages

  • No in-house production/QC
  • Consistency for long-term and multi-site studies
  • Simple media exchange and imaging
  • 3D expertise on demand 

Cost-effective,
convenient models

• • •

Liver Model Comparison

3D InSight™ Liver Microtissues
Self-made PHH spheroids

 
  • Why are 3D InSight™ Liver Microtissues superior to self-made PHH spheroid models?

    Request our white paper:

    "Considerations for implementing commercially produced versus “home-brewed” 3D human liver microtissues for assessment of drug-induced liver injury (DILI), liver function and disease."

    Learn how InSphero’s enhanced 3D Select™ Process delivers advanced 3D liver models for efficient integration into your drug discovery and development workflow. Bypass experimentation with published methods to ensure:

    • Maximum donor compatibility as evidenced by head-to-head method comparison using hepatocytes from six different donors
    • Superior morphology demonstrated by detailed IHC analysis of PHH spheroids and 3D InSight™ Human Liver Microtissues
    • Higher viability confirmed by ATP endpoint analysis

    3Dselect

  • Considerations for Integration of 3D Liver Models

     

      # 3D InSight™Liver Microtissues Self-made PHH Spheroids
    Model development Complete Months to years
    Model availability Weekly delivery in assay-ready format Dependent on in-house resources
    Model consistency Fully QC'd May vary lot to lot; requires internal QC
    Hepatocyte procurement and characterization Pre-qualified donor lots secured for consistency over longitudinal studies Requires in-house purchasing and qualification; availability may vary
    Application-specific model bioengineering Co-culture with Kupffer cells, LSECs, and stellate cells available; ongoing development of complex models Requires additional development, characterization, and QC resources per model
    Endpoint flexibility 3D-optimized DILI & mechanistic toxicity endpoint protocols available Requires protocol development and validation
    Understanding of 3D models and industry impact 8 years of scientific knowledge and commercial proficiency in 3D model production; strategic partnerships with industry, regulatory, and academic influencers Acquired over time; requires investment in staff training and education

Company video

company-video-inspheor