3D InSight™ Rat Liver Microtissues

Primary Sprague-Dawley hepatocyte-derived 3D rat liver models delivering greater physiological relevance for investigative toxicology studies

3D InSight™ Rat Liver Microtissues
  • Leverage scaffold-free 3D microtissue models composed of primary rat hepatocytes and NPCs
  • Predict drug-induced liver injury (DILI) in a 3D in vitro rat model that reflects in vivo species-specific toxicities (e.g., sensitivity to Acetaminophen)
  • Perform mechanistic toxicity studies in an advanced model featuring intact hepatocyte function, bile salt excretion, and inflammatory-mediated toxicity pathways

 

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  • InSphero 3D InSight™ Rat Liver Microtissues are produced and shipped to customers in the United States and Europe. Place your order on Tuesday at 6 PM (CET) three weeks before your desired ship date based on our production schedule. (Please indicate your desired ship date when requesting a quote.) For more detailed information about the order and shipment dates for all InSphero microtissue products, please see our quarterly production schedule.

    3D InSight™ Rat Liver Microtissues
    Production lead time: please inquire

    Catalog # Description
    MT-02-001-01 3D InSight™ Rat Liver Microtissues from primary hepatocytes, monoculture (96x)
    MT-02-001-04 3D InSight™ Rat Liver Microtissues from primary hepatocytes, co-culture with NPCs (96x)
    CS-07-002 Maintenance Medium for Rat liver 500 mL*
    AK-01-001-01 Cytotoxicity Assay Kit (for up to 4 96-well plates)

    For volume discounts (starting at 2 plates) and shipping cost, please contact our sales staff to request a quote. We start rat liver microtissue production runs every three weeks, special formulations upon request.

    *only available together with 3D InSight™ Microtissues

  • Organotypic microtissue composition

    3D InSight™ Rat Liver Microtissues are generated using cryopreserved primary rat hepatocytes and non-parenchymal cells (NPCs) in a ratio designed to reflect the organotypic cell composition of native rat liver. Hepatocytes are polarized and form functional bile-canaliculi. In addition, Kupffer cells are incorporated in the microtissue and display sensitivity to external stimuli, such as bacteria-derived lipopolysaccharide (LPS). The presence of stellate cells helps maintain hepatocyte functionality. No bioartificial scaffold materials are used in the microtissue production process. InSphero 3D InSight™ Rat Liver Microtissues are delivered in our spheroid-optimized assay plate, ready to use in experiments within hours of receipt in your lab.

                                             3D organotypic rat liver microtissue models

     

    Figure 1: Rat Liver Microtissues were stained for glycogen storage (Periodic Acid-Schiff, PAS), and presence of Kupffer cells (CD68 marker) and stellate cells (αSMA marker). Extensive storage of glycogen indicates high metabolic activity, CD68 staining shows incorporation of Kupffer cells in microtissues, and peripheral αSMA staining indicates the presence of stellate cells.

  • Applications

    3D InSight™ Rat Liver Microtissues are an excellent alternative to traditional animal models for:

    • Acute hepatotoxicity
    • Species-specific toxicity testing
    • Mechanistic toxicity testing
      • Inflammation-mediated toxicity (idiosyncratic toxicity)
      • Mitochondrial toxicity
      • Oxidative stress
    • ADME/Tox

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