3D InSight™ HepG2 Liver Microtissues

 Your easiest option for early-stage hepatotoxicity screens

  • The best way to compare previous 2D test results to 3D data
  • HepG2 regains organotypic functionality in 3D-microtissue culture
  • Long term (>10 days) studies of drug effects possible

 

  • Physiologically relevant toxicity testing

    Cultured using InSphero's patent pending process, HepG2 cells form compact, liver-like 3D spheroids. The abundance of close cell-to-cell contacts assured by the scaffold-free microtissue formation results in long life times and high functionality of the HepG2 spheroids. These are capable of providing far more physiologically relevant data than cells in conventional monolayer culture. The ease of use of InSphero's assay-ready microtissues and the compatibility with existing 2D workflows and read-outs makes HepG2 microtissues a perfect choice to upgrade your toxicity screen to 3D. 3D HepG2 microtissues: Delivered in Gravity- TRAP™ plates – ready for you to use!

    Same cell type, improved characteristics

    Compared to monolayer culture, HepG2s in 3D immediately display key morphological and functional characteristics of native hepatocytes:

    • cuboidal cell shape
    • formation of bile canaliculi
    • increased albumin and glutamate production
    • increased CYP450 induction
    • higher drug efflux activity

    3D InSight HepG2 Liver Spheroid characteristics

    Accumulation of CMFDA dye in bile canaliculi, which is actively transported by MRP-2. From D. Müller, A. Koetemann, F. Noor (2011), J Bioengineer & Biomedical Sci (doi:10.4172/2155- 9538.S2-002)

    Metabolically active liver-like models

    The HepG2 cell line is widely used for liver toxicity testing, although the cells lose many liver-specific functions in 2D culture. These are, to a substantial extend, re-gained in 3D, which makes HepG2 microtissues an ideal solution for hepatotoxicity testing. When cultured by InSphero using its scaffold-free technology they even express bile canaliculi and display MRP2 activity. It was never so easy to harness the high reproducibility of a cell line combined with the predictive power of metabolically active liver-like cells.

    3D InSight HepG2 Liver Spheroid metabolic activity

    Electron microscopy of a HepG2 microtissue section. Indicated is the presence of bile canaliculi and tight cell-cell contacts with desmosomes. From J.M. Kelm et al. (2003) Biotechnology and Bioengineering (doi: 10.1002/bit.10655).

    Supplied to you ready-to-use

    3D HepG2 liver microtissues are supplied ready-to-use in the specially designed GravityTRAP™ plate. In this format automated plate readers can easily locate and analyse microtissues. In addition, the plate is designed to facilitate media changes and compound dosing during long term maintenance. The GravityTRAP™ plate is also suitable for standard luminescent and fluorescent assays, e.g. Promega ATP, LDH, GSH and apoptosis assays.
    • Acute toxicity testing
    • Early-stage toxicology screening
    • High-content screening
    • Genotoxicity testing
    • ADME/Tox
  • Catalog # Description
    MT-02-100-01 HepG2 liver microtissues (96x)
    CS-07-100 3D microtissue maintenance medium, for HepG2 (500 mL)

    For pricing and and shipping costs, please request a quote.

     

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