Literature References

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Read about applications of InSphero microtissues and technology in peer-reviewed journal articles.  

Liver Microtissues | Islet Microtissues | Cardiac & Stem Cell |  Cancer/Oncology | Embryotoxicity
Scaffold-free 3D Technology | Body on a Chip | Regenerative Medicine & Tissue Engineering
 

Liver Microtissues

Hendriks DF, Fredriksson Puigvert L, Messner S, Mortiz W, Ingelman-Sundberg M (2016) Hepatic 3D spheroid models for the detection and study of compounds with cholestatic liability.  Sci Rep. 2016 Oct 19;6:35434. doi: 10.1038/srep35434.

Linfeng L., Zhou Q., Voss T.C., Quick K.L., LaBarbera D.V., (2016). High-throughput imaging: Focusing in on drug discovery in 3D. Methods. 2016 Mar 1;96:97-102. doi: 10.1016/j.ymeth.2015.11.013. Epub 2015 Nov 21.

Bruderer R., Bernhardt O.M., Gandhi T., Miladinovic S.M., Cheng L.Y., Messner S., Ehrenberger T., Zanotelli V., Butscheid Y., Escher C., Vitek O., Rinner O., and Reiter L. (2015). Extending the limits of quantitative proteome profiling with data-independent acquisition and application to acetaminophen treated 3D liver microtissues. Mol Cell Proteomics. Feb 27. pii: mcp.M114.044305. [Epub ahead of print]

Kermanizadeh A., Lohr M., Roursqaard M., Messner S., Gunness P., Kelm J.M., Moller P., Ston V., Loft S. (2014). Hepatic toxicology follwing single and multiple exposure of nanomaterials utilising a novel primary human 3D liver microtissue model. Part Fibre Toxicol. Oct 20; 11(1):56. [Epub ahaead of print]

Fruhwurth S., Kovacs W.J., Bittman R., Messner S., Rohrl C., Stangl H. (2014). Differential basolateral-apical distribution of scavenger receptor, class B, type I in cultured cells and the liver. Histochem Cell Biol. Jul 25. [Epub ahead of print]

Alepee N., Bahinski T., Daneshian M., et. al. (2014). State-of-the-art of 3D cultures (organs-on-a-chip) in safety testing and pathophysiology. ALTEX. Jul 14. doi: http://dx.doi.org/10.14573/altex1406111. [Epub ahead of print]

Kratschmar D.V., Messner S., Moritz W. and Odermatt A. (2013). Characterization of a Rat Multi-Cell Type 3D-Liver Microtissue System. J Tissue Sci Eng 4: 130.

Merx V. (2013). "Cell culture: A better brew" Nature International Weekly Journal of Science. 496, 253–258.

Messner S., Agarkova I., Moritz W. and Kelm J.M. (2013). Multi-cell type human liver microtissues for hepatotoxicity testing In: Archives of Toxicology. Springer-Verlag. 209-213.

 

Islet Microtissues

Li G, Wu B, Ward MG, Chong ACN, Mukherjee S, Chen S, Hao M (2016) Multifunctional in vivo imaging of pancreatic islets during diabetes development. Development. 2016 Aug 1; 143(15):e1.2. doi: 10.1242/dev.142372

Bader E, Migliorini A, Gegg M, Moruzzi N, Gerdes J, Roscioni SS, Bakhti M, Brandl E, Irmler M, Beckers J, Aichler M, Feuchtinger A, Leitzinger C, Zischka H, Wang-Sattler R, Jastroch M, Tschöp M, Machicao F, Staiger H, Häring HU, Chmelova H, Chouinard JA, Oskolkov N, Korsgren O, Speier S, Lickert H (2016) Identification of proliferative and mature ß-cells in the islets of Langerhans.  Nature. 2016 Jul 21;535(7612):430-4.

Zuellig R.A., Cavallari G., Gerber P., Tschopp O., Spinas G.A., Moritz W. and Lehmann R.  (2014). Improved physiological properties of gravity-enforced reassembled rat and human pancreatic pseudo-islets. J Tissue Eng Regen Med. Apr 16. doi: 10.1002/term.1891. [Epub ahead of print]

 

Cardiac & Stem Cell-derived Microtissues

Beauchamp P., Moritz W., Kelm J.M., Ullrich N.D., Agarkova I., Anson B.D., Suter T.M., Zuppinger C. (2015). Development and Characterization of a Scaffold-Free 3D Spheroid Model of Induced Pluripotent Stem Cell Derived Human Cardiomyocytes. Tissue Eng Part C Methods. 2015 Aug;21(8):852-61. doi: 10.1089/ten.TEC.2014.0376. Epub 2015 Mar 16.

Rismani Yazdi S., Shadmani A., Bürgel S.C., Misun P.M., Hierlemann A., Frey O. (2015). Adding the "heart" to hanging drop networks for microphysiological multi-tissue experiments. Lab Chip. Sep 24 [Epub ahead of print].

 Suter-Dick L., Alves P.M., Blaauboer B.J., Bremm K.D., Brito C., Coecke S., Flick B., Fowler P., Hescheler J., Ingelman-Sundberg M., Jennings P., Kelm J.M., Manou I., Mistry P., Moretto A., Roth A., Stedman D., van de Water B., and Beilmann M. (2015). Stem cell derived (SCD) systems in toxicology assessment. Stem Cells Dev. Feb 12 [Epub ahead of print]

Kelm J.M., Djonov V., Hoerstrup S.P., Guenter C.I., Ittner L.M., Greve F., Hierlemann A., Perriard J.C., Ehler E. and Fussenegger M. (2006). Tissue-Transplant Fusion and Vascularization of Myocardial Micro-and Macrotissues Implanted into Chicken Embryos and Rats. Tissue Eng. 12, 2541-2553. Winning article of the young investigator award 2006. European Society of Artificial Organs (ESAO).

Kelm, J.M., Ehler E., Nielsen L.K., Schlatter S., Perriard J.C. and Fussenegger M. (2004). Design of Artificial Myocardial Microtissues. Tissue Eng. 10, 201-214.

 

Cancer/Oncology

Huber JM, Amann A, Koeck S, Lorenz E, Kelm JM, Obexer P, Zwierzina H, Gamerith G (2016) Evaluation of assays for drug efficacy in a three-dimensional model of the lung.  J Cancer Res and Clin Oncol. 2016 Sep;142(9):1955-66. doi 10.1007/s00432-016-2198-0. epub 2016 Jul 16.

Falkenberg, N., Höfig I, Rosemann M., Szumielewski J., Richter S., Schorpp K., Hadian K., Aubele M., Atkinson M.J., Anastasov N. (2016). Three-dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer. Cancer Med. 2016 Jan 14. doi: 10.1002/cam4.630. [Epub ahead of print]

Anastasov N., Hofig I., Radulovic V., Strobel S., Salomon M., Lichtenberg J., Rothenaigner I., Hadian K., Kelm J.M., Thirion C., Atkinson M.J. (2015). A 3D-microtissue-based phenotypic screening of radiation resistant tumor cells with synchronized chemotherapeutic treatment.  BMC Cancer. Jun 10. 15:466. doi: 10.1186/s12885-015-1481-9.

Falkenberg N., Anastasov N., Hofig I, Bashkueva K., Hofler H., Rosemann M., Aubele M. (2015). Additive impact of HER2-/PTK6-RNAi on interactions with HER3 or IGF-1R leads to reduced breast cancer progression in vivo. Mol Oncol. Jan 9 (1): 282-94.  doi: 10.1016/j.molonc.2014.08.012. Epub 2014 Sep 6.

Amann A., Zwierzina M., Gamerith G., Bitsche M., Huber J.M., Vogel G.F., Blumer M., Koeck S., Pechriggl E.J., Kelm J.M., Hilbe W. and Zwierzina H. (2014). Development of an innovative 3D cell culture system to study tumour--stroma interactions in non-small cell lung cancer cells. PLoS One.

Thoma C., Zimmermann M., Agarkova I., Kelm J.M. and Krek W. (2014). 3D cell culture systems modeling tumor growth determinants in cancer target discovery. Adv Drug Deliv Rev.

Thoma C., Stroebel S., Rösch N., Calpe B., Krek W. and Kelm J.M. (2013). A High-Throughput–Compatible 3D Microtissue Co-culture System for Phenotypic RNAi Screening Applications. Journal of Biomolecular Screening.

Oberdanner C., Kopish K., Stevenson J., Salomon M., Drewitz M. and Kelm J.M. (2012). Multiplexed Drug Assessment in 3D. Combining Luminescence and Fluorescence Genetic Reporters to Assess Drug Effects. Genetic Engineering & Biotechnology News.

Helbling M.M., Drewitz M., Bieri M., Lotz C., Wyder L., Lehembre F., Moritz W., Lichtenberg J., Regenass U. and Kelm J.M. (2011). Characterization and Drug Sensitivity Testing of HTS-Compatible Cancer Microtissues. American Association of Cancer Research Annual Meeting, Orlando.

Kelm, J.M., Timmins N.E., Brown C.J., Fussenegger M. and Nielsen L.K. (2003). Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. Biotechnol Bioeng 83, 173-80.

 

 

Embryotoxicity Testing

Stroebel S., Lichtenberg J., Moritz W. and Kelm J.M. (2011). Towards Automation of the Embryonic Stem Cell Test Society of Biomolecular Screening Annual Meeting, Orlando.

 

GravityPLUS® and GravityTRAP™ Scaffold-free 3D Technologies

Muoth C, Wichser A, Monopoli M, Correia M, Ehrlich N, Loeschner K, Gallud A, Kucki M, Diener L, Manser P, Jochum W, Wick P, Buerki-Thurnherr T (2016).  A 3D co-culture microtissue model of the human placenta for nanotoxicity assessment. Nanoscale. 2016 Oct 6;8(39):17322-17332.

Huber JM, Amann A, Koeck S, Lorenz E, Kelm JM, Obexer P, Zwierzina H, Gamerith G (2016) Evaluation of assays for drug efficacy in a three-dimensional model of the lung.  J Cancer Res and Clin Oncol. 2016 Sep;142(9):1955-66. doi 10.1007/s00432-016-2198-0. epub 2016 Jul 16.

Falkenberg, N., Höfig I, Rosemann M., Szumielewski J., Richter S., Schorpp K., Hadian K., Aubele M., Atkinson M.J., Anastasov N. (2016). Three-dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer. Cancer Med. 2016 Jan 14. doi: 10.1002/cam4.630. [Epub ahead of print]

Keller L., Wagner Q., Offner D., Eap S., Musset A.-M., Arruebo M., Kelm J.M., Schwinté P., Benkirane-Jessel N. (2015). Integrating Microtissues in Nanofiber Scaffolds for Regenerative NanomedicineMaterials 8, 6863-6867.

Anastasov N., Hofig I., Radulovic V., Strobel S., Salomon M., Lichtenberg J., Rothenaigner I., Hadian K., Kelm J.M., Thirion C., Atkinson M.J. (2015). A 3D-microtissue-based phenotypic screening of radiation resistant tumor cells with synchronized chemotherapeutic treatment.  BMC Cancer. Jun 10. 15:466. doi: 10.1186/s12885-015-1481-9.

Falkenberg N., Anastasov N., Hofig I, Bashkueva K., Hofler H., Rosemann M., Aubele M. (2015). Additive impact of HER2-/PTK6-RNAi on interactions with HER3 or IGF-1R leads to reduced breast cancer progression in vivo. Mol Oncol. Jan 9 (1): 282-94.  doi: 10.1016/j.molonc.2014.08.012. Epub 2014 Sep 6.

Rimann M., Laternser S., Gvozdenovic A., Muff R., Fuchs B., Kelm J.M., Graf-Hausner U. (2014). An in vitro osteosarcoma 3D microtissue model for drug development. J Biotechnol. Sep 16; 189C:129-135. doid: 10.1016/j.jbiotec.2014.09.005. [Epub ahead of print]

Amann A., Zwierzina M., Gamerith G., Bitsche M., Huber J.M., Vogel G.F., Blumer M., Koeck S., Pechriggl E.J., Kelm J.M., Hilbe W. and Zwierzina H. (2014). Development of an innovative 3D cell culture system to study tumour--stroma interactions in non-small cell lung cancer cells. PLoS One.

Thoma C., Zimmermann M., Agarkova I., Kelm J.M. and Krek W. (2014). 3D cell culture systems modeling tumor growth determinants in cancer target discovery. Adv Drug Deliv Rev.

Thoma C., Stroebel S., Rösch N., Calpe B., Krek W. and Kelm J.M. (2013). A High-Throughput–Compatible 3D Microtissue Co-culture System for Phenotypic RNAi Screening Applications. Journal of Biomolecular Screening.

Merx V. (2013). "Cell culture: A better brew" Nature International Weekly Jounral of Science. 496, 253–258.

Kelm J.M. and Lichtenberg J.(2013). 3D Cell Culture for Compound De-Risking Innovations in Pharmaceutical Technology, Zurich.

Kelm J.M. and Fussenegger M. (2004). Microscale tissue engineering using gravity-enforced cell assembly. Trends Biotechnol. 22, 195-202.

Kelm J.M., Lorber V., Snedeker J.G., Schmidt D., Broggini-Tenzer A., Weisstanner M., Odermatt B., Mol A., Zünd G. and Hoerstrup S.P. (2010). A Novel Concept for Scaffold-Free Vessel Tissue Engineering: Self-Assembly of Microtissue Building Blocks J Biotech 148, 46-55.

Kelm J.M. and Fussenegger M. (2010) Scaffold-free cell delivery for use in regenerative medicine Adv Drug Delivery Rev 62, 753-64.

Diaz Sanchez-Bustamante C., Kelm J.M., Mitta B. and Fussenegger M. (2006). Heterologous Protein Production Capacity of Mammalian Cells in 2D and 3D cultures. Biotechnol Bioeng. 93, 169-180.

 

 

Body on a Chip

Rismani Yazdi S., Shadmani A., Bürgel S.C., Misun P.M., Hierlemann A., Frey O. (2015). Adding the "heart" to hanging drop networks for microphysiological multi-tissue experiments. Lap Chip. Sep 24 [Epub ahead of print].

Kim J., Fluri D.A., Marchan R., Boonen K., Mohanty S., Singh P., Hammad S., Landuyt B., Hengstler J.G., Kelm J.M., Hierlemann A., and Frey O. (2015). 3D spherical microtissues and microfluidic technology for multi-tissue experiments and analysis. J Biotechnol. Jan 12. pii: S0168-1656(15)00012-7. doi: 10.1016/j.jbiotec.2015.01.003. [Epub ahead of print]

Kim J., Fluri D.A., Kelm J.M., Hierlemann A., and Frey O. (2014). 96-well format microfluidic platform for parallel interconnection of multiple multicellular spheroids.  J Lab Autom. Dec 18. pii: 2211068214564056 [Epub ahead of print]

Frey O., Misun P.M., Fluri D.A., Hengstler J.G. and Hierlemann A.  (2014). Reconfigurable microfluidic hanging drop network for multi-tissue interaction and analysis. Nat Commun. Jun 30; 5:4250. doi: 10.1038/ncomms5250.

  

 

Regenerative Medicine & Tissue Engineering

Keller L., Wagner Q., Offner D., Eap S., Musset A.-M., Arruebo M., Kelm J.M., Schwinté P., Benkirane-Jessel N. (2015). Integrating Microtissues in Nanofiber Scaffolds for Regenerative Nanomedicine. Materials 8, 6863-6867.

Rimann M., Laternser S., Gvozdenovic A., Muff R., Fuchs B., Kelm J.M., Graf-Hausner U. (2014). An in vitro osteosarcoma 3D microtissue model for drug development. J Biotechnol. Sep 16; 189C:129-135. doid: 10.1016/j.jbiotec.2014.09.005. [Epub ahead of print]

 Kelm J.M. and Fussenegger M. (2010) Scaffold-free cell delivery for use in regenerative medicine Adv Drug Delivery Rev 62, 753-64.

 Diaz Sanchez-Bustamante C., Kelm J.M., Egermann E., Djonov V. and Fussenegger M. (2007) Ectopic expression of DFosB mediates transdifferention of adipose-like spheroids into osteo-like microtissues. Tissue Eng 14, 1377-94.

Kelm J.M., Djonov V., Ittner L.M., Born W., Hoerstrup S.P. and Fussenegger M. (2006). Design of Custom-Shaped Vascularized Tissues Using Microtissue Spheroids as Minimal Building Units. Tissue Eng. 12, 2151-2160.

Kelm J.M., Diaz Sanchez-Bustamante C., Ehler E., Hoerstrup S.P., Djonov D., Ittner L.M. and Fussenegger M.(2005). VEGF profiling and angiogenesis in human microtissues. J. Biotechnol. 121, 86-101.

Kelm, J.M., Kramer B.P., Gonzalez-Nicolini V., Ley B. and Fussenegger M. (2004). Synergies of Microtissue Design, Viral Transduction and Adjustable Transgene Expression for Regenerative Medicine.” Biotechnol Appl Biochem. 39, 3-16.

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