Technical Protocols

Handling, viability, and energy | Liver-specific | Islet-specific | Histology | RNA Isolation


Make the most of your 3D InSight™ Microtissues with technical protocols for 3D-cell-based assays!

When you purchase 3D InSight™ Microtissues, you gain access to a library of 3D-optimized technical protocols that contain step-by-step instructions and tips to help you achieve the highest quality and most relevant data using 3D InSight™ microtissues. Here are some of our most useful 3D-optimized protocols, which you can request from your InSphero representative.  Check back frequently for updates on available protocols, as we are continually partnering with gold-standard assay providers to develop new, reliable methods using our microtissues.  This email address is being protected from spambots. You need JavaScript enabled to view it.

 Technical Protocol RNA isolation from 3D Microtissues using the Promega Maxwell 16 System

Handling, viability, and energy protocols (non-tissue-specific)

  • Microtissue handling (TP007)
  • LDH-based assessment of microtissue health using the Promega CytoTox-ONE™ Assay (TP003)
  • ATP measurement in 3D InSight™ Microtissues using Promega CellTiter-Glo® 3D Cell Viability Assay (TP001)

Liver-specific protocols

  • Measurement of Alpha GST release from 3D InSight™ Liver Microtissues (TP004)

Islet-specific protocols

  • Assessment of β-cell function in 3D InSight™ Human Islet Microtissues (TP013)
  • Assessment of α-cell function in 3D InSight™ Human Islet Microtissues (TP015)

Histology protocols

  • Microtissue-processing-for-histology (TP006)
  • Whole mount microtissue staining (TP008)

RNA Isolation protocols

  • RNA-isolation-from-3D-Microtissues-using-the-Promega-Maxwell-16-System (TP 009b)
  • RNA-isolation-from-3D-Microtissues-using-the-Promega-ReliaPrep-RNA-Tissue-Miniprep-System (TP009a)


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