Technical Protocols

Technical Protocols

Posted in Technical Protocols

Make the most of your microtissues!
Whether you're using our assay-ready microtissues or growing spheroids with our GravityPLUS™ Culture and Assay Platform, we've assembled some of our most useful 3D-optimized protocols to help you achieve the highest quality and most relevant data. Check back frequently for more 3D InSight™, as we are continually partnering with gold-standard assay providers to develop new, reliable methods using our 3D InSight™ microtissues.  This email address is being protected from spambots. You need JavaScript enabled to view it.

Handling, viability, and energy | Liver-specific | Islet-specific | Histology | RNA Isolation

3-D optimized technical protocols

 

Handling, viability, and energy protocols (non-tissue-specific)

  • Microtissue handling in GravityTRAP™ Plates (TP007)
  • LDH-based assessment of microtissue health using the Promega CytoTox-ONE™ Assay (TP003)
  • ATP measurement in 3D Microtissues using Promega CellTiter-Glo® 3D Cell Viability Assay (TP001)

Liver-specific protocols

  • Measurement of Alpha GST release from 3D InSight™ Liver Microtissues (TP004)

Islet-specific protocols

  • Assessment of β-cell function in 3D InSight™ Human Islet Microtissues (TP013)
  • Assessment of α-cell function in 3D InSight™ Human Islet Microtissues (TP015)

Histology protocols

  • Microtissue-processing-for-histology (TP006)
  • Whole mount microtissue staining in GravityTRAP™ plates (TP008)

RNA Isolation protocols

  • RNA-isolation-from-3D-Microtissues-using-the-Promega-Maxwell-16-System (TP 009b)
  • RNA-isolation-from-3D-Microtissues-using-the-Promega-ReliaPrep-RNA-Tissue-Miniprep-System (TP009a)

 

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